Echinoderm eggs and embryos: procurement and culture.

TitleEchinoderm eggs and embryos: procurement and culture.
Publication TypeJournal Article
Year of Publication2004
AuthorsFoltz KR, Adams NL, Runft LL
JournalMethods Cell Biol
Volume74
Pagination39-74
Date Published2004
ISSN0091-679X
KeywordsAnimals, Aquaculture, Cell Culture Techniques, Echinodermata, Embryo, Nonmammalian, Female, Larva, Male, Models, Animal, Ovum, Reproduction
Abstract

The protocols outlined here hopefully will provide researchers with healthy, beautiful echinoderm oocytes, eggs, and embryos for experimental use. The large size of echinoderm oocytes and eggs, the ease with which they can be manipulated, and (in many species) their optical clarity, make them an ideal model system for studying not only the events specific to oocyte maturation and fertilization, but also for investigating more general questions regarding cell cycle regulation in an in vivo system. The quick rate at which development proceeds after fertilization to produce transparent embryos and larva makes the echinoderm an advantageous organism for studying deuterostome embryogenesis. Continued use of the echinoderms as model systems will undoubtedly uncover exciting answers to questions regarding fertilization, cell cycle regulation, morphogenesis, and how developmental events are controlled.

Alternate JournalMethods Cell Biol.
PubMed ID15575602