A molecular signature for anastasis, recovery from the brink of apoptotic cell death.

TitleA molecular signature for anastasis, recovery from the brink of apoptotic cell death.
Publication TypeJournal Article
Year of Publication2017
AuthorsSun G, Guzman E, Balasanyan V, Conner CM, Wong K, Zhou HRobin, Kosik KS, Montell DJ
JournalJ Cell Biol
Volume216
Issue10
Pagination3355-3368
Date Published2017 10 02
ISSN1540-8140
KeywordsApoptosis, Cell Movement, Cell Proliferation, HeLa Cells, Humans, Snail Family Transcription Factors, Wound Healing
Abstract

<p>During apoptosis, executioner caspase activity has been considered a point of no return. However, recent studies show that cells can survive caspase activation following transient apoptotic stimuli, a process called anastasis. To identify a molecular signature, we performed whole-transcriptome RNA sequencing of untreated, apoptotic, and recovering HeLa cells. We found that anastasis is an active, two-stage program. During the early stage, cells transition from growth-arrested to growing. In the late stage, HeLa cells change from proliferating to migratory. Recovering cells also exhibited prolonged elevation of proangiogenic factors. Strikingly, some early-recovery mRNAs, including Snail, were elevated first during apoptosis, implying that dying cells poise to recover, even while under apoptotic stress. Snail was also required for recovery. This study reveals similarities in the anastasis genes, pathways, and cell behaviors to those activated in wound healing and identifies a repertoire of potential targets for therapeutic manipulation.</p>

DOI10.1083/jcb.201706134
Alternate JournalJ. Cell Biol.
PubMed ID28768686
PubMed Central IDPMC5626555
Grant ListDP1 CA195760 / CA / NCI NIH HHS / United States