A Cdc42-mediated supracellular network drives polarized forces and Drosophila egg chamber extension.

TitleA Cdc42-mediated supracellular network drives polarized forces and Drosophila egg chamber extension.
Publication TypeJournal Article
Year of Publication2020
AuthorsPopkova A, Stone OJ, Chen L, Qin X, Liu C, Liu J, Belguise K, Montell DJ, Hahn KM, Rauzi M, Wang X
JournalNat Commun
Volume11
Issue1
Pagination1921
Date Published2020 04 21
ISSN2041-1723
KeywordsActins, Actomyosin, Animals, Anisotropy, Cell Adhesion, Cell Polarity, Cytoskeleton, Drosophila, Drosophila Proteins, Epithelium, Female, Glutathione Transferase, Green Fluorescent Proteins, GTP-Binding Proteins, Myosin Type II, Oogenesis, Optogenetics, Pseudopodia, RNA Interference
Abstract

Actomyosin supracellular networks emerge during development and tissue repair. These cytoskeletal structures are able to generate large scale forces that can extensively remodel epithelia driving tissue buckling, closure and extension. How supracellular networks emerge, are controlled and mechanically work still remain elusive. During Drosophila oogenesis, the egg chamber elongates along the anterior-posterior axis. Here we show that a dorsal-ventral polarized supracellular F-actin network, running around the egg chamber on the basal side of follicle cells, emerges from polarized intercellular filopodia that radiate from basal stress fibers and extend penetrating neighboring cell cortexes. Filopodia can be mechanosensitive and function as cell-cell anchoring sites. The small GTPase Cdc42 governs the formation and distribution of intercellular filopodia and stress fibers in follicle cells. Finally, our study shows that a Cdc42-dependent supracellular cytoskeletal network provides a scaffold integrating local oscillatory actomyosin contractions at the tissue scale to drive global polarized forces and tissue elongation.

DOI10.1038/s41467-020-15593-2
Alternate JournalNat Commun
PubMed ID32317641
PubMed Central IDPMC7174421
Grant ListR01 GM046425 / GM / NIGMS NIH HHS / United States
R35 GM122596 / GM / NIGMS NIH HHS / United States