A thermogenetics protocol for detecting gap junction channels in egg chambers.

TitleA thermogenetics protocol for detecting gap junction channels in egg chambers.
Publication TypeJournal Article
Year of Publication2021
AuthorsMiao G, Montell D
JournalSTAR Protoc
Volume2
Issue1
Pagination100269
Date Published2021 Mar 19
ISSN2666-1667
Abstract

In this protocol, we took a "thermogenetics" approach to detect functional gap junction channels between cells in egg chambers. We expressed the thermosensitive cation channel TrpA1-A in the germline using Gal4, and a fluorescent [Ca] sensor (GCaMP6), in all follicle cells using the LexA/LexAop system. If gap junctions connect germ cells and follicle cells, we expect a temperature-dependent TRPA1-A-dependent cation influx into the germline from the culture medium to result in a GCaMP signal in follicle cells. For complete details on the use and execution of this protocol, please refer to Miao et al. (2020).

DOI10.1016/j.xpro.2020.100269
Alternate JournalSTAR Protoc
PubMed ID33490985
PubMed Central IDPMC7811055