|Title||A thermogenetics protocol for detecting gap junction channels in egg chambers.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Miao G, Montell D|
|Date Published||2021 Mar 19|
In this protocol, we took a "thermogenetics" approach to detect functional gap junction channels between cells in egg chambers. We expressed the thermosensitive cation channel TrpA1-A in the germline using Gal4, and a fluorescent [Ca] sensor (GCaMP6), in all follicle cells using the LexA/LexAop system. If gap junctions connect germ cells and follicle cells, we expect a temperature-dependent TRPA1-A-dependent cation influx into the germline from the culture medium to result in a GCaMP signal in follicle cells. For complete details on the use and execution of this protocol, please refer to Miao et al. (2020).
|Alternate Journal||STAR Protoc|
|PubMed Central ID||PMC7811055|