Shining light on Drosophila oogenesis: live imaging of egg development.

TitleShining light on Drosophila oogenesis: live imaging of egg development.
Publication TypeJournal Article
Year of Publication2011
AuthorsHe L, Wang X, Montell DJ
JournalCurr Opin Genet Dev
Volume21
Issue5
Pagination612-9
Date Published2011 Oct
ISSN1879-0380
KeywordsAnimals, Cell Movement, Drosophila melanogaster, Epithelium, Female, Oogenesis, Ovary
Abstract

<p>Drosophila oogenesis is a powerful model for the study of numerous questions in cell and developmental biology. In addition to its longstanding value as a genetically tractable model of organogenesis, recently it has emerged as an excellent system in which to combine genetics and live imaging. Rapidly improving ex vivo culture conditions, new fluorescent biosensors and photo-manipulation tools, and advances in microscopy have allowed direct observation in real time of processes such as stem cell self-renewal, collective cell migration, and polarized mRNA and protein transport. In addition, entirely new phenomena have been discovered, including revolution of the follicle within the basement membrane and oscillating assembly and disassembly of myosin on a polarized actin network, both of which contribute to elongating this tissue. This review focuses on recent advances in live-cell imaging techniques and the biological insights gleaned from live imaging of egg chamber development.</p>

DOI10.1016/j.gde.2011.08.011
Alternate JournalCurr Opin Genet Dev
PubMed ID21930372
PubMed Central IDPMC6824908
Grant ListR01 GM046425 / GM / NIGMS NIH HHS / United States