Psidin, a conserved protein that regulates protrusion dynamics and cell migration.

TitlePsidin, a conserved protein that regulates protrusion dynamics and cell migration.
Publication TypeJournal Article
Year of Publication2011
AuthorsKim JHoon, Cho A, Yin H, Schafer DA, Mouneimne G, Simpson KJ, Nguyen K-V, Brugge JS, Montell DJ
JournalGenes Dev
Volume25
Issue7
Pagination730-41
Date Published2011 Apr 01
ISSN1549-5477
KeywordsActin Depolymerizing Factors, Animals, Blood Proteins, Cell Line, Tumor, Cell Movement, Cell Surface Extensions, Drosophila melanogaster, Drosophila Proteins, Female, Gene Expression Regulation, Gene Silencing, Green Fluorescent Proteins, Humans, Mutation, Ovary, Tropomyosin
Abstract

<p>Dynamic assembly and disassembly of actin filaments is a major driving force for cell movements. Border cells in the Drosophila ovary provide a simple and genetically tractable model to study the mechanisms regulating cell migration. To identify new genes that regulate cell movement in vivo, we screened lethal mutations on chromosome 3R for defects in border cell migration and identified two alleles of the gene psidin (psid). In vitro, purified Psid protein bound F-actin and inhibited the interaction of tropomyosin with F-actin. In vivo, psid mutations exhibited genetic interactions with the genes encoding tropomyosin and cofilin. Border cells overexpressing Psid together with GFP-actin exhibited altered protrusion/retraction dynamics. Psid knockdown in cultured S2 cells reduced, and Psid overexpression enhanced, lamellipodial dynamics. Knockdown of the human homolog of Psid reduced the speed and directionality of migration in wounded MCF10A breast epithelial monolayers, whereas overexpression of the protein increased migration speed and altered protrusion dynamics in EGF-stimulated cells. These results indicate that Psid is an actin regulatory protein that plays a conserved role in protrusion dynamics and cell migration.</p>

DOI10.1101/gad.2028611
Alternate JournalGenes Dev.
PubMed ID21406550
PubMed Central IDPMC3070935
Grant ListU54-GM064346 / GM / NIGMS NIH HHS / United States
R01 GM073164 / GM / NIGMS NIH HHS / United States
R01 GM67222 / GM / NIGMS NIH HHS / United States
R01 GM73164 / GM / NIGMS NIH HHS / United States
U54 GM064346 / GM / NIGMS NIH HHS / United States
R01 GM067222 / GM / NIGMS NIH HHS / United States