Tissue elongation requires oscillating contractions of a basal actomyosin network.

TitleTissue elongation requires oscillating contractions of a basal actomyosin network.
Publication TypeJournal Article
Year of Publication2010
AuthorsHe L, Wang X, Tang HLam, Montell DJ
JournalNat Cell Biol
Volume12
Issue12
Pagination1133-42
Date Published2010 Dec
ISSN1476-4679
KeywordsActomyosin, Animals, Animals, Genetically Modified, Cell Adhesion, Cell Physiological Phenomena, Drosophila melanogaster, Embryo, Nonmammalian, Female, Organogenesis, Ovarian Follicle
Abstract

<p>Understanding how molecular dynamics leads to cellular behaviours that ultimately sculpt organs and tissues is a major challenge not only in basic developmental biology but also in tissue engineering and regenerative medicine. Here we use live imaging to show that the basal surfaces of Drosophila follicle cells undergo a series of directional, oscillating contractions driven by periodic myosin accumulation on a polarized actin network. Inhibition of the actomyosin contractions or their coupling to extracellular matrix (ECM) blocked elongation of the whole tissue, whereas enhancement of the contractions exaggerated it. Myosin accumulated in a periodic manner before each contraction and was regulated by the small GTPase Rho, its downstream kinase, ROCK, and cytosolic calcium. Disrupting the link between the actin cytoskeleton and the ECM decreased the amplitude and period of the contractions, whereas enhancing cell-ECM adhesion increased them. In contrast, disrupting cell-cell adhesions resulted in loss of the actin network. Our findings reveal a mechanism controlling organ shape and an experimental model for the study of the effects of oscillatory actomyosin activity within a coherent cell sheet.</p>

DOI10.1038/ncb2124
Alternate JournalNat. Cell Biol.
PubMed ID21102441
PubMed Central IDPMC3056411
Grant ListR01 GM073164-07 / GM / NIGMS NIH HHS / United States
R01 GM073164 / GM / NIGMS NIH HHS / United States
R01 GM046425-10 / GM / NIGMS NIH HHS / United States
S10 RR024550 / RR / NCRR NIH HHS / United States
R01 GM46425 / GM / NIGMS NIH HHS / United States
R01 GM046425 / GM / NIGMS NIH HHS / United States
R01 GM046425-09 / GM / NIGMS NIH HHS / United States
GM73164 / GM / NIGMS NIH HHS / United States
R01 GM073164-06 / GM / NIGMS NIH HHS / United States