Cellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell imaging.

TitleCellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell imaging.
Publication TypeJournal Article
Year of Publication2007
AuthorsPrasad M, Montell DJ
JournalDev Cell
Volume12
Issue6
Pagination997-1005
Date Published2007 Jun
ISSN1534-5807
KeywordsAnimals, Cell Movement, Cells, Cultured, Diagnostic Imaging, Drosophila melanogaster, Drosophila Proteins, Epithelial Cells, ErbB Receptors, Female, Immunoenzyme Techniques, Male, Microscopy, Fluorescence, Ovary, Receptor Protein-Tyrosine Kinases, Receptors, Notch
Abstract

<p>Border cells in the Drosophila ovary originate within an epithelium, detach from it, invade neighboring nurse cells, and migrate as a coherent cluster. This migration has served as a useful genetic model for understanding epithelial cell motility. The prevailing model of growth factor-mediated chemotaxis in general, and of border cells in particular, posits that receptor activation promotes cellular protrusion at the leading edge. Here we report the time-lapse video imaging of border cell migration, allowing us to test this model. Reducing the activities of the guidance receptors EGFR and PVR did not result in the expected inhibition of protrusion, but instead resulted in protrusion in all directions. In contrast, reduction in Notch activity resulted in failure of the cells to detach from the epithelium without affecting direction sensing. These observations provide new insight into the cellular dynamics and molecular mechanisms of cell migration in vivo.</p>

DOI10.1016/j.devcel.2007.03.021
Alternate JournalDev. Cell
PubMed ID17543870
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States
GM73164 / GM / NIGMS NIH HHS / United States
GM46425 / GM / NIGMS NIH HHS / United States