Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary.

TitleAnalysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary.
Publication TypeJournal Article
Year of Publication2006
AuthorsWang X, Bo J, Bridges T, Dugan KD, Pan T-chi, Chodosh LA, Montell DJ
JournalDev Cell
Volume10
Issue4
Pagination483-95
Date Published2006 Apr
ISSN1534-5807
KeywordsAnimals, CCAAT-Enhancer-Binding Proteins, CD8 Antigens, Cell Movement, Cells, Cultured, Drosophila, Drosophila Proteins, Female, Gene Expression Profiling, Mice, Mutation, Oligonucleotide Array Sequence Analysis, Ovary, Transcription Factors
Abstract

<p>Cell migration contributes to normal development and homeostasis as well as to pathological processes such as inflammation and tumor metastasis. Previous genetic screens have revealed signaling pathways that govern follicle cell migrations in the Drosophila ovary, but few downstream targets of the critical transcriptional regulators have been identified. To characterize the gene expression profile of two migratory cell populations and identify Slbo targets, we purified border cells and centripetal cells expressing the mouse CD8 antigen and carried out whole-genome microarray analysis. Genes predicted to control actin dynamics and the endocytic and secretory pathways were overrepresented in the migratory cell transcriptome. Mutations in five genes, including ttk, failed to complement previously isolated mutations that cause cell migration defects in mosaic clones. Functional analysis revealed a role for the Notch-activating protease Kuzbanian in border cell migration and identified Tie as a guidance receptor for the border cells.</p>

DOI10.1016/j.devcel.2006.02.003
Alternate JournalDev. Cell
PubMed ID16580993
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States
U54GM064346 / GM / NIGMS NIH HHS / United States
GC10988-119467 / / PHS HHS / United States