Requirement for Par-6 and Bazooka in Drosophila border cell migration.

TitleRequirement for Par-6 and Bazooka in Drosophila border cell migration.
Publication TypeJournal Article
Year of Publication2004
AuthorsPinheiro EM, Montell DJ
JournalDevelopment
Volume131
Issue21
Pagination5243-51
Date Published2004 Nov
ISSN0950-1991
KeywordsAnimals, Cadherins, CCAAT-Enhancer-Binding Proteins, Cell Adhesion, Cell Movement, Cell Polarity, Drosophila melanogaster, Drosophila Proteins, Epithelial Cells, Female, Gene Expression, Integrins, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mutation, Myosin Heavy Chains, Ovary, Protein Transport, Proteins, Receptors, Interleukin, RNA Interference, Transcription Factors
Abstract

<p>Polarized epithelial cells convert into migratory invasive cells during a number of developmental processes, as well as when tumors metastasize. Much has been learned recently concerning the molecules and mechanisms that are responsible for generating and maintaining epithelial cell polarity. However, less is known about what becomes of epithelial polarity proteins when various cell types become migratory and invasive. Here, we report the localization of several apical epithelial proteins, Par-6, Par-3/Bazooka and aPKC, during border cell migration in the Drosophila ovary. All of these proteins remained asymmetrically distributed throughout migration. Moreover, depletion of either Par-6 or Par-3/Bazooka by RNAi resulted in disorganization of the border cell cluster and impaired migration. The distributions of several transmembrane proteins required for migration were abnormal following Par-6 or Par-3/Bazooka downregulation, possibly accounting for the migration defects. Taken together, these results indicate that cells need not lose apical/basal polarity in order to invade neighboring tissues and in some cases even require such polarity for proper motility.</p>

DOI10.1242/dev.01412
Alternate JournalDevelopment
PubMed ID15456726
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States