A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration.

TitleA role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration.
Publication TypeJournal Article
Year of Publication2004
AuthorsGeisbrecht ER, Montell DJ
JournalCell
Volume118
Issue1
Pagination111-25
Date Published2004 Jul 09
ISSN0092-8674
KeywordsAnimals, Caspase Inhibitors, Cell Movement, Cells, Cultured, Contractile Proteins, Drosophila, Drosophila Proteins, Female, Genes, Insect, Inhibitor of Apoptosis Proteins, Microfilament Proteins, Models, Biological, Mutation, Ovary, Profilins, rac GTP-Binding Proteins
Abstract

<p>Border cell migration in the Drosophila ovary is a relatively simple and genetically tractable model for studying the conversion of epithelial cells to migratory cells. Like many cell migrations, border cell migration is inhibited by a dominant-negative form of the GTPase Rac. To identify new genes that function in Rac-dependent cell motility, we screened for genes that when overexpressed suppressed the migration defect caused by dominant-negative Rac. Overexpression of the Drosophila inhibitor of apoptosis 1 (DIAP1), which is encoded by the thread (th) gene, suppressed the migration defect. Moreover, loss-of-function mutations in th caused migration defects but, surprisingly, did not cause apoptosis. Mutations affecting the Dark protein, an activator of the upstream caspase Dronc, also rescued RacN17 migration defects. These results indicate an apoptosis-independent role for DIAP1-mediated Dronc inhibition in Rac-mediated cell motility.</p>

DOI10.1016/j.cell.2004.06.020
Alternate JournalCell
PubMed ID15242648
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States
GM46425 / GM / NIGMS NIH HHS / United States