Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

TitleActivated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.
Publication TypeJournal Article
Year of Publication2004
AuthorsSilver DL, Naora H, Liu J, Cheng W, Montell DJ
JournalCancer Res
Volume64
Issue10
Pagination3550-8
Date Published2004 May 15
ISSN0008-5472
KeywordsAnimals, Cell Movement, Cytoskeletal Proteins, DNA-Binding Proteins, Epithelial Cells, Female, Focal Adhesion Kinase 1, Focal Adhesion Protein-Tyrosine Kinases, Focal Adhesions, Humans, Mice, NIH 3T3 Cells, Ovarian Neoplasms, Paxillin, Phosphoproteins, Phosphorylation, Protein-Tyrosine Kinases, STAT3 Transcription Factor, Trans-Activators
Abstract

<p>Constitutive activation of the Janus-activated kinase/signal transducer and activator of transcription (STAT) pathway promotes the proliferation and survival of cancer cells in culture and is associated with various cancers, including those of the ovary. We found that constitutively activated STAT3 levels correlated with aggressive clinical behavior of ovarian carcinoma specimens. Furthermore, inhibition of STAT3 reduced the motility of ovarian cancer cells in vitro. Surprisingly, we found that activated STAT3 localized not only to nuclei but also to focal adhesions in these cells. Activated STAT3 coimmunoprecipitated with phosphorylated paxillin and focal adhesion kinase and required paxillin and Src for its localization to focal adhesions. These results suggest that Janus-activated kinase/STAT signaling may contribute to ovarian cancer cell invasiveness.</p>

DOI10.1158/0008-5472.CAN-03-3959
Alternate JournalCancer Res.
PubMed ID15150111
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States
GM46425 / GM / NIGMS NIH HHS / United States