The transmembrane protein Off-track associates with Plexins and functions downstream of Semaphorin signaling during axon guidance.

TitleThe transmembrane protein Off-track associates with Plexins and functions downstream of Semaphorin signaling during axon guidance.
Publication TypeJournal Article
Year of Publication2001
AuthorsWinberg ML, Tamagnone L, Bai J, Comoglio PM, Montell D, Goodman CS
JournalNeuron
Volume32
Issue1
Pagination53-62
Date Published2001 Oct 11
ISSN0896-6273
KeywordsAlleles, Animals, Axons, Cell Adhesion Molecules, Neuronal, COS Cells, DNA, Antisense, Drosophila, Drosophila Proteins, Gene Expression Regulation, Developmental, In Vitro Techniques, Ligands, Membrane Proteins, Molecular Sequence Data, Mutagenesis, Insertional, Nerve Tissue Proteins, Phenotype, Protein-Tyrosine Kinases, Receptors, Cell Surface, Receptors, Fibroblast Growth Factor, Semaphorins, Signal Transduction
Abstract

<p>The Plexin family of transmembrane proteins appears to function as repulsive receptors for most if not all Semaphorins. Here, we use genetic and biochemical analysis in Drosophila to show that the transmembrane protein Off-track (OTK) associates with Plexin A, the receptor for Sema 1a, and that OTK is a component of the repulsive signaling response to Semaphorin ligands. In vitro, OTK associates with Plexins. In vivo, mutations in the otk gene lead to phenotypes resembling those of loss-of-function mutations of either Sema1a or PlexA. The otk gene displays strong genetic interactions with Sema1a and PlexA, suggesting that OTK and Plexin A function downstream of Sema 1a.</p>

DOI10.1016/s0896-6273(01)00446-9
Alternate JournalNeuron
PubMed ID11604138
Grant ListE.1129 / / Telethon / Italy
R01 AG063907 / AG / NIA NIH HHS / United States
HD21294 / HD / NICHD NIH HHS / United States