Multiple Ras signals pattern the Drosophila ovarian follicle cells.

TitleMultiple Ras signals pattern the Drosophila ovarian follicle cells.
Publication TypeJournal Article
Year of Publication1997
AuthorsLee T, Montell DJ
JournalDev Biol
Volume185
Issue1
Pagination25-33
Date Published1997 May 01
ISSN0012-1606
KeywordsAnimals, CCAAT-Enhancer-Binding Proteins, Cell Lineage, DNA-Binding Proteins, Drosophila, Embryonic Induction, Female, Nuclear Proteins, Ovarian Follicle, ras Proteins, Signal Transduction
Abstract

<p>During Drosophila oogenesis, spatially restricted activity of the TORPEDO receptor tyrosine kinase first recruits follicle cells adjacent to the oocyte to a posterior cell fate and then specifies dorsal follicle cells. Another receptor tyrosine kinase, BREATHLESS, stimulates migration of the anterior follicle cells known as border cells. Since Ras is known to mediate many receptor tyrosine kinase effects, we have investigated the role of Ras in follicle cell fate determination, differentiation, and migration throughout oogenesis. Early ectopic Ras activity induced transient expression of posterior follicle cell markers in anterior follicle cells, but did not inhibit anterior differentiation. Later ectopic Ras activity inhibited anterior follicle cell differentiation but did not induce posterior marker expression. Complete transformation of anterior follicle cells to posterior follicle cells required early ectopic Ras activity in egg chambers where terminal differentiation of anterior cells was inhibited. These results suggest that, in vivo as in vitro, Ras can have diverse effects on different cells, but, in addition, Ras activity can have different effects on the same cells at different stages in their development.</p>

DOI10.1006/dbio.1997.8537
Alternate JournalDev. Biol.
PubMed ID9169047
Grant ListR01 AG063907 / AG / NIA NIH HHS / United States
GM46425 / GM / NIGMS NIH HHS / United States