Cell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesis.

TitleCell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesis.
Publication TypeJournal Article
Year of Publication1996
AuthorsMurphy AM, Montell DJ
JournalJ Cell Biol
Volume133
Issue3
Pagination617-30
Date Published1996 May
ISSN0021-9525
KeywordsActins, Animals, Base Sequence, Biological Transport, Cell Movement, Cloning, Molecular, Cytoplasm, Drosophila, Drosophila Proteins, Female, Germ Cells, GTP Phosphohydrolases, GTP-Binding Proteins, Insect Hormones, Membrane Proteins, Molecular Sequence Data, Oogenesis, Ovum, rac GTP-Binding Proteins, Sequence Homology, Amino Acid
Abstract

<p>The Rho subfamily of GTPases has been shown to regulate cellular morphology. We report the discovery of a new member of the Rho family, named RhoL, which is equally similar to Rac, Rho, and Cdc42. Expression of a dominant-negative RhoL transgene in the Drosophila ovary caused nurse cells to collapse and fuse together. Mutant forms of Cdc42 mimicked this effect. Expression of constitutively active RhoL led to nurse cell subcortical actin breakdown and disruption of nurse cell-follicle cell contacts, followed by germ cell apoptosis. In contrast, Rac activity was specifically required for migration of a subset of follicle cells called border cells. All three activities were necessary for normal transfer of nurse cell cytoplasm to the oocyte. These results suggest that Rho protein activities have cell type-specific effects on morphogenesis.</p>

DOI10.1083/jcb.133.3.617
Alternate JournalJ. Cell Biol.
PubMed ID8636236
PubMed Central IDPMC2120826
Grant ListR296M46425 / / PHS HHS / United States