slow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP.

Titleslow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP.
Publication TypeJournal Article
Year of Publication1992
AuthorsMontell DJ, Rorth P, Spradling AC
JournalCell
Volume71
Issue1
Pagination51-62
Date Published1992 Oct 02
ISSN0092-8674
KeywordsAmino Acid Sequence, Animals, Base Sequence, CCAAT-Enhancer-Binding Proteins, Cell Movement, Cloning, Molecular, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Female, Genes, Insect, Microscopy, Electron, Molecular Sequence Data, Nuclear Proteins, Oogenesis, Ovary, Recombinant Fusion Proteins, Restriction Mapping, Transcription Factors
Abstract

<p>During Drosophila oogenesis six to ten follicle cells, the border cells, undergo a dramatic and stereotypic migration through the developing egg chamber. We identified four independent P element insertion mutations that specifically blocked border cell migration. They defined a single, novel locus that was named slow border cells (slbo), because hypomorphic alleles caused delayed onset of the migration. Laser ablation of the border cells, or failure of their migration, caused improper morphogenesis of the micropyle, the egg-shell structure through which the sperm enters at fertilization. The slbo locus was found to encode a product homologous to the CCAAT/enhancer-binding protein (C/EBP), a basic region-leucine zipper transcription factor. Drosophila C/EBP may be required for the expression of gene products mediating border cell migration.</p>

DOI10.1016/0092-8674(92)90265-e
Alternate JournalCell
PubMed ID1394432
Grant List / / Howard Hughes Medical Institute / United States
R29GM46425 / GM / NIGMS NIH HHS / United States