Laser ablation studies of the role of the Drosophila oocyte nucleus in pattern formation.

TitleLaser ablation studies of the role of the Drosophila oocyte nucleus in pattern formation.
Publication TypeJournal Article
Year of Publication1991
AuthorsMontell DJ, Keshishian H, Spradling AC
JournalScience
Volume254
Issue5029
Pagination290-3
Date Published1991 Oct 11
ISSN0036-8075
KeywordsAnimals, Cell Nucleus, Cell Polarity, Drosophila, Egg Shell, Genes, Laser Therapy, Microsurgery, Morphogenesis, Mutation, Oocytes, Oogenesis
Abstract

<p>Somatic and germline cells interact during oogenesis to establish the pattern axes of the Drosophila eggshell and embryo. The role of the oocyte nucleus in pattern formation was tested with the use of laser ablation. Ablation in stage 6 to 9 egg chambers caused partial or complete ventralization of the eggshell, phenotypes similar to those of eggs produced by gurken or torpedo females. Accumulation of vasa protein at the posterior pole of treated oocytes was also disrupted. Thus the oocyte nucleus is required as late as stage 9 for dorsoventral patterning within the follicle cells and for polar plasm assembly in the oocyte.</p>

DOI10.1126/science.1925585
Alternate JournalScience
PubMed ID1925585
Grant List / / Howard Hughes Medical Institute / United States