Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration.

TitleMechanical feedback through E-cadherin promotes direction sensing during collective cell migration.
Publication TypeJournal Article
Year of Publication2014
AuthorsCai D, Chen S-C, Prasad M, He L, Wang X, Choesmel-Cadamuro V, Sawyer JK, Danuser G, Montell DJ
JournalCell
Volume157
Issue5
Pagination1146-59
Date Published2014 May 22
ISSN1097-4172
KeywordsAnimals, Biomechanical Phenomena, Cadherins, Cell Adhesion, Cell Movement, Chemotaxis, Cytological Techniques, Drosophila melanogaster, Drosophila Proteins, Female, Molecular Sequence Data, Ovary, rac GTP-Binding Proteins
Abstract

<p>E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-specific RNAi, photoactivatable Rac, and morphodynamic profiling, to interrogate how E-cadherin contributes to collective migration of cells between other cells. Using the Drosophila ovary as a model, we found that adhesion between border cells and their substrate, the nurse cells, functions in a positive feedback loop with Rac and actin assembly to stabilize forward-directed protrusion and directionally persistent movement. Adhesion between individual border cells communicates direction from the lead cell to the followers. Adhesion between motile cells and polar cells holds the cluster together and polarizes each individual cell. Thus, E-cadherin is an integral component of the guidance mechanisms that orchestrate collective chemotaxis in vivo.</p>

DOI10.1016/j.cell.2014.03.045
Alternate JournalCell
PubMed ID24855950
PubMed Central IDPMC4118667
Grant ListGM71868 / GM / NIGMS NIH HHS / United States
R01 GM073164 / GM / NIGMS NIH HHS / United States
GM46425 / GM / NIGMS NIH HHS / United States
U54 GM064346 / GM / NIGMS NIH HHS / United States
R01 GM046425 / GM / NIGMS NIH HHS / United States
GM64346 / GM / NIGMS NIH HHS / United States
GM73164 / GM / NIGMS NIH HHS / United States
R01 GM071868 / GM / NIGMS NIH HHS / United States