Live Imaging of Border Cell Migration in Drosophila.

TitleLive Imaging of Border Cell Migration in Drosophila.
Publication TypeJournal Article
Year of Publication2016
AuthorsDai W, Montell DJ
JournalMethods Mol Biol
Volume1407
Pagination153-68
Date Published2016
ISSN1940-6029
KeywordsAnimals, Cell Movement, Cell Tracking, Drosophila, Gene Expression, Genes, Reporter, Microscopy, Confocal, Oocytes, Oogenesis, Ovum, Time-Lapse Imaging
Abstract

<p>Border cells are a cluster of cells that migrate from the anterior tip of the Drosophila egg chamber to the border of the oocyte in stage 9. They serve as a useful model to study collective cell migration in a native tissue environment. Here we describe a protocol for preparing ex vivo egg chamber cultures from transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.</p>

DOI10.1007/978-1-4939-3480-5_12
Alternate JournalMethods Mol. Biol.
PubMed ID27271901
Grant ListR01 GM046425 / GM / NIGMS NIH HHS / United States
R01 GM073164 / GM / NIGMS NIH HHS / United States