CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo.

TitleCasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo.
Publication TypeJournal Article
Year of Publication2016
AuthorsDing AXun, Sun G, Argaw YG, Wong JO, Easwaran S, Montell DJ
JournalElife
Volume5
Date Published2016 04 08
ISSN2050-084X
KeywordsAnimals, Apoptosis, Caspase 3, Cell Survival, Cytological Techniques, Drosophila, Spatio-Temporal Analysis
Abstract

<p>Caspase-3 carries out the executioner phase of apoptosis, however under special circumstances, cells can survive its activity. To document systematically where and when cells survive caspase-3 activation in vivo, we designed a system, CasExpress, which drives fluorescent protein expression, transiently or permanently, in cells that survive caspase-3 activation in Drosophila. We discovered widespread survival of caspase-3 activity. Distinct spatial and temporal patterns emerged in different tissues. Some cells activated caspase-3 during their normal development in every cell and in every animal without evidence of apoptosis. In other tissues, such as the brain, expression was sporadic both temporally and spatially and overlapped with periods of apoptosis. In adults, reporter expression was evident in a large fraction of cells in most tissues of every animal; however the precise patterns varied. Inhibition of caspase activity in wing discs reduced wing size demonstrating functional significance. The implications of these patterns are discussed.</p>

DOI10.7554/eLife.10936
Alternate JournalElife
PubMed ID27058168
PubMed Central IDPMC4865370
Grant ListR01 GM046425 / GM / NIGMS NIH HHS / United States
R21 EY022498 / EY / NEI NIH HHS / United States