Modeling and analysis of collective cell migration in an in vivo three-dimensional environment.

TitleModeling and analysis of collective cell migration in an in vivo three-dimensional environment.
Publication TypeJournal Article
Year of Publication2016
AuthorsCai D, Dai W, Prasad M, Luo J, Gov NS, Montell DJ
JournalProc Natl Acad Sci U S A
Volume113
Issue15
PaginationE2134-41
Date Published2016 Apr 12
ISSN1091-6490
KeywordsAnimals, Cell Size, Chemotactic Factors, Chemotaxis, Drosophila, Extracellular Matrix, Models, Biological, Oocytes, Surface Properties
Abstract

<p>A long-standing question in collective cell migration has been what might be the relative advantage of forming a cluster over migrating individually. Does an increase in the size of a collectively migrating group of cells enable them to sample the chemical gradient over a greater distance because the difference between front and rear of a cluster would be greater than for single cells? We combined theoretical modeling with experiments to study collective migration of the border cells in-between nurse cells in the Drosophila egg chamber. We discovered that cluster size is positively correlated with migration speed, up to a particular point above which speed plummets. This may be due to the effect of viscous drag from surrounding nurse cells together with confinement of all of the cells within a stiff extracellular matrix. The model predicts no relationship between cluster size and velocity for cells moving on a flat surface, in contrast to movement within a 3D environment. Our analyses also suggest that the overall chemoattractant profile in the egg chamber is likely to be exponential, with the highest concentration in the oocyte. These findings provide insights into collective chemotaxis by combining theoretical modeling with experimentation. </p>

DOI10.1073/pnas.1522656113
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID27035964
PubMed Central IDPMC4839456
Grant ListR01 GM046425 / GM / NIGMS NIH HHS / United States
S10 OD010610 / OD / NIH HHS / United States