Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement.

TitleBorder Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement.
Publication TypeJournal Article
Year of Publication2015
AuthorsPrasad M, Wang X, He L, Cai D, Montell DJ
JournalMethods Mol Biol
Volume1328
Pagination89-97
Date Published2015
ISSN1940-6029
KeywordsAnimals, Cell Movement, Drosophila melanogaster, Female, Microscopy, Fluorescence, Molecular Biology, Oogenesis, Organ Culture Techniques, Ovum
Abstract

<p>Border cell migration in the Drosophila ovary has emerged as a genetically tractable model for studying collective cell movement. Over many years border cell migration was exclusively studied in fixed samples due to the inability to culture stage 9 egg chambers in vitro. Although culturing late-stage egg chambers was long feasible, stage 9 egg chambers survived only briefly outside the female body. We identified culture conditions that support stage 9 egg chamber development and sustain complete migration of border cells ex vivo. This protocol enables one to compare the dynamics of egg chamber development in wild-type and mutant egg chambers using time-lapse microscopy and taking advantage of a multiposition microscope with a motorized imaging stage. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.</p>

DOI10.1007/978-1-4939-2851-4_6
Alternate JournalMethods Mol. Biol.
PubMed ID26324431
PubMed Central IDPMC4762686
Grant ListR01 GM073164 / GM / NIGMS NIH HHS / United States
GM73164 / GM / NIGMS NIH HHS / United States