Title | Differential regulation of the sleep homeostat by circadian and arousal inputs |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Ni JD, Gurav AS, Liu W, Ogunmowo TH, Hackbart H, Elsheikh A, Verdegaal AA, Montell C |
Journal | eLife |
Volume | 8:e40487 |
Date Published | 2019 Feb 05 |
ISSN | 2050-084X |
Abstract | One output arm of the sleep homeostat in appears to be a group of neurons with projections to the dorsal fan-shaped body (dFB neurons) of the central complex in the brain. However, neurons that regulate the sleep homeostat remain poorly understood. Using neurogenetic approaches combined with Ca imaging, we characterized synaptic connections between dFB neurons and distinct sets of upstream sleep-regulatory neurons. One group of the sleep-promoting upstream neurons is a set of circadian pacemaker neurons that activates dFB neurons via direct glutaminergic excitatory synaptic connections. Opposing this population, a group of arousal-promoting neurons downregulates dFB axonal output with dopamine. Co-activating these two inputs leads to frequent shifts between sleep and wake states. We also show that dFB neurons release the neurotransmitter GABA and inhibit octopaminergic arousal neurons. We propose that dFB neurons integrate synaptic inputs from distinct sets of upstream sleep-promoting circadian clock neurons, and arousal neurons. |
DOI | 10.7554/eLife.40487 |
Alternate Journal | Elife |
PubMed ID | 30719975 |
PubMed Central ID | PMC6363385 |
Grant List | DC007864 / / National Institute on Deafness and Other Communication Disorders / EY008117 / / National Eye Institute / |