Conserved modules required for TRP function

TitleConserved modules required for TRP function
Publication TypeJournal Article
Year of Publication2021
AuthorsChen Z, Kerwin M, Keenan O, Montell C
JournalJ Neurosci
Date Published2021 Jun 07
ISSN1529-2401
Abstract

TRP channels are broadly required in animals for sensory physiology. To provide insights into regulatory mechanisms, the structures of many TRPs have been solved. This has led to new models, some of which have been tested Here, using the classical TRP required for visual transduction, we uncovered structural requirements for channel function in photoreceptor cells. Using a combination of molecular genetics, field recordings, protein expression analysis, and molecular modeling, we interrogated roles for the S4-S5 linker and the TRP domain, and revealed mutations in the S4-S5 linker that impair channel opening or closing. We also uncovered differential requirements for the two highly conserved motifs in the TRP domain for activation and protein stability. By performing genetic complementation, we found an intra-subunit interaction between the S4-S5 linker and the S5 segment that contributes to activation. This analysis highlights key structural requirements for TRP channel opening, closing, folding and for intra-subunit interactions in a native context- photoreceptor cells.The importance of TRP channels for sensory biology and human health has motivated tremendous effort in trying to understand the roles of the structural motifs essential for their activation, inactivation and protein folding. In the current work, we have exploited the unique advantages of the visual system to reveal mechanistic insights into TRP channel function in a native system-photoreceptor cells. Using a combination of electrophysiology (field recordings), cell biology and molecular modeling, we have revealed roles of key motifs for activation, inactivation and protein folding of TRP .

DOI10.1523/JNEUROSCI.0200-21.2021
Alternate JournalJ Neurosci
PubMed ID34099505
PubMed Central IDPMC8265800
Grant ListP40 OD010949 / OD / NIH HHS / United States
R01 EY010852 / EY / NEI NIH HHS / United States