Lysosomal localization of TRPML3 depends on TRPML2 and the mucolipidosis-associated protein TRPML1

TitleLysosomal localization of TRPML3 depends on TRPML2 and the mucolipidosis-associated protein TRPML1
Publication TypeJournal Article
Year of Publication2006
AuthorsVenkatachalam K, Hofmann T, Montell C
JournalJ Biol Chem
Volume281
Pagination17517-27
Date Published2006 Jun 23
ISSN0021-9258
KeywordsAnimals, Cell Line, Cell Membrane, Humans, Lysosomes, Mice, Mucolipidoses, NIH 3T3 Cells, Transient Receptor Potential Channels, TRPM Cation Channels
Abstract

Mucolipidosis type IV is an autosomal recessive lysosomal storage disorder characterized by severe neurodegeneration, achlorhydria, and visual impairments such as corneal opacity and strabismus. The disease arises due to mutations in a group 2 transient receptor potential (TRP)-related cation channel, TRPML1. Mammals encode two additional TRPML proteins named TRPML2 and TRPML3. Information regarding the propensity of these proteins to multimerize, their subcellular distribution and mechanisms that regulate their trafficking are limited. Here we demonstrate that TRPMLs interact to form homo- and heteromultimers. Moreover, the presence of either TRPML1 or TRPML2 specifically influences the spatial distribution of TRPML3. TRPML1 and TRPML2 homomultimers are lysosomal proteins, whereas TRPML3 homomultimers are in the endoplasmic reticulum. However, TRPML3 localizes to lysosomes when coexpressed with either TRPML1 or TRPML2 and is comparably mislocalized when lysosomal targeting of TRPML1 and TRPML2 is disrupted. Conversely, TRPML3 does not cause retention of TRPML1 or TRPML2 in the endoplasmic reticulum. These data demonstrate that there is a hierarchy controlling the subcellular distributions of the TRPMLs such that TRPML1 and TRPML2 dictate the localization of TRPML3 and not vice versa.

DOI10.1074/jbc.M600807200
Alternate JournalJ. Biol. Chem.
PubMed ID16606612
Grant ListEY10852 / EY / NEI NIH HHS / United States
R01 EY010852-13 / EY / NEI NIH HHS / United States