Title | Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | Lee S-J, Xu H, Montell C |
Journal | Proc Natl Acad Sci U S A |
Volume | 101 |
Pagination | 11874-9 |
Date Published | 2004 Aug 10 |
ISSN | 0027-8424 |
Keywords | Animals, Animals, Genetically Modified, beta-Adrenergic Receptor Kinases, Cyclic AMP-Dependent Protein Kinases, Drosophila, Drosophila Proteins, Electrophysiology, Eye Proteins, G-Protein-Coupled Receptor Kinase 1, G-Protein-Coupled Receptor Kinase 3, Phosphorylation, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, Vision, Ocular |
Abstract | A feature shared between Drosophila rhodopsin and nearly all other G protein-coupled receptors is agonist-dependent protein phosphorylation. Despite extensive analyses of Drosophila phototransduction, the identity and function of the rhodopsin kinase (RK) have been elusive. Here, we provide evidence that G protein-coupled receptor kinase 1 (GPRK1), which is most similar to the beta-adrenergic receptor kinases, G protein-coupled receptor kinase 2 (GRK2) and GRK3, is the fly RK. We show that GPRK1 is enriched in photoreceptor cells, associates with the major Drosophila rhodopsin, Rh1, and phosphorylates the receptor. As is the case with mammalian GRK2 and GRK3, Drosophila GPRK1 includes a C-terminal pleckstrin homology domain, which binds to phosphoinositides and the Gbetagamma subunit. To address the role of GPRK1, we generated transgenic flies that expressed higher and lower levels of RK activity. Those flies with depressed levels of RK activity displayed a light response with a much larger amplitude than WT. Conversely, the amplitude of the light response was greatly suppressed in transgenic flies expressing abnormally high levels of RK activity. These data point to an evolutionarily conserved role for GPRK1 in modulating the amplitude of the visual response. |
DOI | 10.1073/pnas.0402205101 |
Alternate Journal | Proc. Natl. Acad. Sci. U.S.A. |
PubMed ID | 15289614 |
PubMed Central ID | PMC511067 |
Grant List | EY08117 / EY / NEI NIH HHS / United States |