Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites

TitleElimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites
Publication TypeJournal Article
Year of Publication1984
AuthorsMontell C, Berk AJ
JournalNucleic Acids Res
Volume12
Pagination3821-7
Date Published1984 May 11
ISSN0305-1048
KeywordsAdenoviruses, Human, Base Sequence, Coliphages, Dinucleoside Phosphates, HeLa Cells, Humans, Mutation, Nucleic Acid Hybridization, Oligonucleotides, RNA Splicing, RNA, Messenger
Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site.

Alternate JournalNucleic Acids Res.
PubMed ID6587322
PubMed Central IDPMC318792
Grant ListAI 07116 / AI / NIAID NIH HHS / United States
CA 25235 / CA / NCI NIH HHS / United States
GM 07104 / GM / NIGMS NIH HHS / United States