Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA

TitleInhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA
Publication TypeJournal Article
Year of Publication1983
AuthorsMontell C, Fisher EF, Caruthers MH, Berk AJ
JournalNature
Volume305
Pagination600-5
Date Published1983 Oct 13-19
ISSN0028-0836
KeywordsAdenoviruses, Human, Base Sequence, DNA, Genes, Viral, HeLa Cells, Humans, Mutation, Poly A, RNA, RNA, Messenger, RNA, Viral, Transcription, Genetic
Abstract

A single U leads to G transversion in the 3' consensus sequence AAUAAA of the adenovirus early region 1A gene was constructed and the effect of this mutation on processing of the 3' end of the nuclear early region 1A RNAs was analysed. The results demonstrate that the intact AAUAAA is not required for RNA polyadenylation but is required for the cleavage step preceding polyadenylation to occur efficiently.

Alternate JournalNature
PubMed ID6194440
Grant ListAI 07116 / AI / NIAID NIH HHS / United States
CA 25235 / CA / NCI NIH HHS / United States