Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6.

TitleAntioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6.
Publication TypeJournal Article
Year of Publication2013
AuthorsNicklisch, SCT, Das, S, Rodriguez, NRMartinez, Waite, JH, Israelachvili, JN
JournalBiotechnol Prog
Date Published2013 Nov-Dec
KeywordsAmino Acid Sequence, Animals, Antioxidants, Muscle Proteins, Mytilus, Oxidation-Reduction, Recombinant Proteins

Mytilus foot protein type 6 (mfp-6) is crucial for maintaining the reducing conditions needed for optimal wet adhesion in marine mussels. In this report, we describe the expression and production of a recombinant Mytilus californianus foot protein type 6 variant 1 (rmfp-6.1) fused with a hexahistidine affinity tag in Escherichia coli and its purification by affinity chromatography. Recombinant mfp-6 showed high purification yields of 5-6 mg L(-1) cell culture and excellent solubility in low pH buffers that retard oxidation of its many thiol groups. Purified rmfp-6.1 protein showed high 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity when compared with vitamin C. Using the highly sensitive surface forces apparatus (SFA) technique to measure interfacial surface forces in the nano-Newton range, we show that rmfp-6.1 is also able to rescue the oxidation-dependent adhesion loss of mussel foot protein 3 (mfp-3) at pH 3. The adhesion rescue is related to a reduction of dopaquinone back to 3,4-dihydroxyphenyl-l-alanine in mfp-3, which is the reverse reaction observed during the detrimental enzymatic browning process in fruits and vegetables. Broadly viewed, rmfp-6.1 has potential as a versatile antioxidant for applications ranging from personal products to antispoilants for perishable foods during processing and storage. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1587-1593, 2013.

Alternate JournalBiotechnol. Prog.
PubMed ID24106182
PubMed Central IDPMC3864665
Grant ListR01 DE 018468 / DE / NIDCR NIH HHS / United States
R01 DE018468 / DE / NIDCR NIH HHS / United States