JoVE protocols
Behavior
Temperature Sensation
- Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
- Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
Locomotion, Circadian Rhythm and Sleep Behavior
- Methods to Assay Drosophila Behavior
- Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila
- High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
- Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae
- Quantitative Measurement of the Immune Response and Sleep in Drosophila
Learning and Memory
- Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration
- Operant Learning of Drosophila at the Torque Meter
- Appetitive Associative Olfactory Learning in Drosophila Larvae
Aggression
Taste and Feeding
- Functional Analysis of the Larval Feeding Circuit in Drosophila
- Proboscis Extension Response (PER) Assay in Drosophila
- A Single-fly Assay for Foraging Behavior in Drosophila
- Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
Odor-driven Behavior
- Visually Mediated Odor Tracking During Flight in Drosophila
- A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila
- High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
Ethanol Sensitivity
Lifespan, Exercise and Neurodegenerative Effects on Behavior
- Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining
- Measurement of Lifespan in Drosophila melanogaster
- Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
Controlling & Measuring Neuronal Activity
- Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
- Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
- Preparation of Drosophila Central Neurons for in situ Patch Clamping
- Whole Cell Recordings from Brain of Adult Drosophila
- Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
- Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae
- Loading Drosophila Nerve Terminals with Calcium Indicators
Biochemistry and Molecular Biology
- Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)
- Recombineering Homologous Recombination Constructs in Drosophila
- RNAi Interference by dsRNA Injection into Drosophila Embryos
- Purification of Transcripts and Metabolites from Drosophila Heads
- Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe
Imaging, Staining and Cell Biology
Adult Visual System
- Single Drosophila Ommatidium Dissection and Imaging
- Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
- Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
Larval and Pupal Nervous System
- Drosophila Larval NMJ Immunohistochemistry
- Dissection of Larval CNS in Drosophila Melanogaster
- Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
- Visualization of Proprioceptors in Drosophila Larvae and Pupae
- Drosophila Pupal Abdomen Immunohistochemistry
- In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
- In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
Brain and CNS
- Ex vivo Culturing of Whole, Developing Drosophila Brains
- Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
- An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila
- Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila
- Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
Cell Isolation from Flies
- Dissection of Oenocytes from Adult Drosophila melanogaster
- Laser Capture Microdissection of Drosophila Peripheral Neurons
- Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
Cell Culture
- Preparation of Drosophila S2 cells for Light Microscopy
- Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae