Ciona genetics.

TitleCiona genetics.
Publication TypeJournal Article
Year of Publication2011
AuthorsVeeman MT, Chiba S, Smith WC
JournalMethods Mol Biol
Date Published2011
KeywordsAnimals, Chromosome Mapping, Cloning, Molecular, Cryopreservation, DNA, DNA Mutational Analysis, Female, Fertilization in Vitro, Genetic Techniques, Hybridization, Genetic, Larva, Male, Microinjections, Mutation, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Urochordata

Ascidians, such as Ciona, are invertebrate chordates with simple embryonic body plans and small, relatively non-redundant genomes. Ciona genetics is in its infancy compared to many other model systems, but it provides a powerful method for studying this important vertebrate outgroup. Here we give basic methods for genetic analysis of Ciona, including protocols for controlled crosses both by natural spawning and by the surgical isolation of gametes; the identification and propagation of mutant lines; and strategies for positional cloning.

Alternate JournalMethods Mol. Biol.
PubMed ID21805273
PubMed Central IDPMC3149854
Grant ListR01 HD038701-10 / HD / NICHD NIH HHS / United States