Characterization of a beta-nerve growth factor expression vector for mammalian cells

TitleCharacterization of a beta-nerve growth factor expression vector for mammalian cells
Publication TypeJournal Article
Year of Publication1993
AuthorsClegg DO
JournalGene
Volume130
Issue2
Pagination291-6
Date Published1993 Aug 25
ISSN0378-1119
KeywordsAnimals, Cells, Cultured, Genetic Vectors, L Cells (Cell Line), Mice, Nerve Growth Factors, PC12 Cells, Plasmids, Promoter Regions, Genetic, Repetitive Sequences, Nucleic Acid, RNA, Messenger, Sarcoma Viruses, Murine, Transfection
Abstract

A mammalian expression vector that directs expression of murine beta-nerve growth factor (beta-NGF) from a murine sarcoma virus long terminal repeat (LTR) promoter element was constructed and characterized. The vector, designated pLTRSNGF, was stably transfected into murine L-cells, and beta-NGF mRNA and protein levels were quantified and compared to endogenous levels in control L-cells. Transfection of pLTRSNGF resulted in an approximate doubling of both beta-NGF mRNA and mature beta-NGF protein secreted into the media. Transfection of pLTRSNGF into rat PC 12 cells resulted in colonies of autocrine-differentiating cells that extended dense networks of neurites in the absence of added NGF, indicating that the beta-NGF produced from the vector is biologically active.

Alternate JournalGene
PubMed ID8395451