Laminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons

TitleLaminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons
Publication TypeJournal Article
Year of Publication2001
AuthorsCulley B, Murphy J, Babaie J, Nguyen D, Pagel A, Rousselle P, Clegg DO
JournalNeuroscience Letters
Volume301
Issue2
Pagination83-6
Date Published2001 Mar 30
ISSN0304-3940
KeywordsAnimals, Brain, Cell Adhesion Molecules, Cells, Cultured, Chick Embryo, Extracellular Matrix, Ganglia, Parasympathetic, Ganglia, Spinal, Microscopy, Fluorescence, Neurites, Retina, Spinal Cord
Abstract

Laminin-5 (Ln-5) is an essential component of epithelial basal laminae that is also expressed in the developing nervous system. Here we use a convenient, simple and reproducible in vitro fluorescent assay to assess the neurite outgrowth promoting activity of purified Ln-5. Embryonic chick neurons from dorsal root ganglia, ciliary ganglia, and (to a lesser extent) retina extended neurites on Ln-5, but the neurite outgrowth promoting activity was not as great as that of Ln-1 or Ln-2. Neurons from diencephalon, telencephalon, and spinal cord did not respond to Ln-5.

Alternate JournalNeurosci. Lett.
PubMed ID11248428
Grant ListEY09736 / EY / NEI NIH HHS / United States