Title | Laminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons |
Publication Type | Journal Article |
Year of Publication | 2001 |
Authors | Culley B, Murphy J, Babaie J, Nguyen D, Pagel A, Rousselle P, Clegg DO |
Journal | Neuroscience Letters |
Volume | 301 |
Issue | 2 |
Pagination | 83-6 |
Date Published | 2001 Mar 30 |
ISSN | 0304-3940 |
Keywords | Animals, Brain, Cell Adhesion Molecules, Cells, Cultured, Chick Embryo, Extracellular Matrix, Ganglia, Parasympathetic, Ganglia, Spinal, Microscopy, Fluorescence, Neurites, Retina, Spinal Cord |
Abstract | Laminin-5 (Ln-5) is an essential component of epithelial basal laminae that is also expressed in the developing nervous system. Here we use a convenient, simple and reproducible in vitro fluorescent assay to assess the neurite outgrowth promoting activity of purified Ln-5. Embryonic chick neurons from dorsal root ganglia, ciliary ganglia, and (to a lesser extent) retina extended neurites on Ln-5, but the neurite outgrowth promoting activity was not as great as that of Ln-1 or Ln-2. Neurons from diencephalon, telencephalon, and spinal cord did not respond to Ln-5. |
Alternate Journal | Neurosci. Lett. |
PubMed ID | 11248428 |
Grant List | EY09736 / EY / NEI NIH HHS / United States |