|lin-12 and glp-1 are required zygotically for early embryonic cellular interactions and are regulated by maternal GLP-1 signaling in Caenorhabditis elegans.
|Year of Publication
|Moskowitz IP, Rothman JH
|Animals, Body Patterning, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Lineage, Chemokine CCL4, Chemokines, CC, Chimera, Embryonic Development, Embryonic Induction, Female, Gene Expression Regulation, Developmental, Helminth Proteins, Laser Therapy, Ligands, Macrophage Inflammatory Proteins, Membrane Glycoproteins, Membrane Proteins, Models, Biological, Proteins, Receptors, Cell Surface, Receptors, Notch, Signal Transduction, Zygote
Cell-cell interactions mediated by LIN-12 and GLP-1, members of the LNG (LIN-12, Notch, GLP-1) family of receptors, are required to specify numerous cell fates during development of the nematode Caenorhabditis elegans. Maternally expressed GLP-1 participates in two of at least four sequential inductive interactions that specify the fates of early embryonic descendants of the AB founder cell. We report that GLP-1 and LIN-12, and apparently their ligand, LAG-2, as well as a downstream component, LAG-1, are required in the latter two inductions. We find that LAG-2 is expressed in the signaling cells and LIN-12 is expressed in cells receiving the inductions, consistent with their proposed roles as ligand and receptor, respectively. Furthermore, we report that maternal GLP-1 activity is required (1) to repress early zygotic lag-2 expression and (2) to activate zygotic lin-12 expression in the early embryo. The patterning of both receptor and ligand expression by maternal GLP-1 signaling establishes competence for the zygotic LNG-mediated cellular interactions and localizes these interactions to the appropriate cells. We propose that activation of maternal GLP-1 regulates zygotic lin-12 and lag-2 expression by a regulatory mechanism analogous to that described for the post-embryonic gonad.
|GM 48137 / GM / NIGMS NIH HHS / United States